The Egyptian Society of Pediatric Allergy and Immunology (ESPAI)The Egyptian Journal of Pediatric Allergy and Immunology1687-164217220191015Updates on hereditary angioedema in pediatrics51605398810.21608/ejpa.2019.53988ENDaliaEl-GhoneimyPediatric Allergy and Immunlogy Unit, Ain Shams University0000-0002-3322-7821Journal Article20191001https://ejpai.journals.ekb.eg/article_53988_039d81ed6ca057c3c6d3dd5fac2623a4.pdfThe Egyptian Society of Pediatric Allergy and Immunology (ESPAI)The Egyptian Journal of Pediatric Allergy and Immunology1687-164217220191015Pertussis seroimmunity in mother-neonate pairs and other pediatric age groups from Egypt61685398910.21608/ejpa.2019.53989ENElhamHossnyProfessor of Pediatrics
Pediatric Allergy and Immunology Unit, Children’s Hospital, Ain Shams University, Cairo, EgyptDaliaEl-GhoneimyPediatric Allergy and Immunlogy Unit, Ain Shams University0000-0002-3322-7821DinaSolimanClinical pathology department, Ain Shams UniversityAlzahraaTaherMinistry of Health Public hospitalsAhmedSheriefMinistry of Public
Health HospitalsJournal Article20191017Background: Despite the widespread availability of 2 classes of effective<br />vaccines, whole cell and acellular, pertussis has resurged as a serious<br />public health problem. We sought to investigate the pertussis immune status<br />of mother-neonate pairs and children in our country where pertussis<br />vaccination is obligatory. Methods: This cross-sectional study included 75<br />healthy full-term neonates and their mothers, 100 infants (2-24 months), 170<br />children (2-12 years) and 80 adolescents (12-18 years). Serum pertussis<br />IgG was measured in all enrolled subjects. A positive titre was defined as<br />>24 U/ml. Results: Positive pertussis IgG levels were detected in 69 of the<br />mothers (92%), in 63 of their newborns (84%). Seroimmunity to pertussis<br />was positively noted in 55% of infants, 82.2% of preschool children, 77.5%<br />of school-aged children and 75% in adolescents. Serum pertussis IgG titers<br />among the neonates showed a significant positive correlation with the<br />maternal titers (P=0.00001). Higher rates of pertussis seroimmunity was<br />observed among residents in urban and suburban areas as compared to<br />those living in rural areas (P<0.05) . Conclusion: This pilot study may<br />suggest the presence of sufficient pertussis seroimmunity rates in the studied<br />age groups. Still, there were some failures in immune acquisition probably<br />due to inefficient vaccination in some localities or waning of immunity with<br />age. Wider scale studies would allow better insight into the pertussis<br />immune status in our country and hence the need for booster immunization.https://ejpai.journals.ekb.eg/article_53989_20a6760c25430749f0925e0ef7951703.pdfThe Egyptian Society of Pediatric Allergy and Immunology (ESPAI)The Egyptian Journal of Pediatric Allergy and Immunology1687-164217220191001Association of T helper type 2 cytokines with sensitization to food in pediatric atopic dermatitis patients69765399010.21608/ejpa.2019.53990ENIlonaPaulauskaitėCentre for Innovative Medicine, Children‘s
Hospital, Lithuania.AudronėEidukaitėAffiliate of Vilnius University Hospital Santaros Klinikos, LithuaniaOdilijaRudzevičienėAffiliate of Vilnius University Hospital Santaros Klinikos, LithuaniaRasaOrentaitėAffiliate of
Vilnius University
Hospital Santaros
Klinikos, Lithuania.Journal Article20190408Background: childhood atopic dermatitis (AD) most commonly presents<br />with sensitization to environmental allergens. Presence of food allergenspecific<br />IgE is common in childhood and does not always correlate with<br />clinical symptoms, which in children usually affect the skin and may<br />exacerbate the course of AD. Exposure to an allergen in the gastrointestinal<br />tract activates Th2 immune reactions. Objective: with this study we wanted<br />to compare blood and stool Th2 cytokine concentrations and fecal<br />calprotectin (FC) value in pediatric AD with (eAD) and without (iAD)<br />sensitization to food. Methods: 51 children with AD were enrolled in the<br />study. 57% (n=29) had food allergen specific IgE and comprised eAD<br />group, 43% (n=22) – iAD group. Blood and stool were tested for IL-4, IL-5,<br />IL-13 concentrations using an enzyme linked immunosorbent assay. Stool<br />samples were tested for FC concentrations. Results: iAD had significantly<br />higher blood and stool IL-4 values than eAD: 2.82 pg/ml vs. 0, p=0.005;<br />2.98 pg/ml vs. 0, p=0.007, respectively. There was no difference in IL-5 and<br />IL-13 blood and stool concentrations between the groups. Children with AD<br />had significantly higher FC values, compared to healthy controls: 36.5<br />mg/kg vs. 6.45 mg/kg, p=0.018. FC was slightly higher in eAD group than<br />iAD, but the difference was not significant: 38.5 mg/kg vs. 25.0 mg/kg,<br />p=0.861 . Conclusions: sensitization to food is not significantly associated<br />with Th2 cytokines in pediatric AD patients. Increase in FC values is<br />characteristic to AD, but not sensitization to food.https://ejpai.journals.ekb.eg/article_53990_e4b254f943d1efa2e11985c3fdf50650.pdfThe Egyptian Society of Pediatric Allergy and Immunology (ESPAI)The Egyptian Journal of Pediatric Allergy and Immunology1687-164217220191001CXCR 3 expression on CD4+T cells and in renal tissue of pediatric systemic lupus erythematosus patients77855399110.21608/ejpa.2019.53991ENMagidIbrahimPediatrics Department, Faculty of Medicine,
Ain Shams University, Cairo, Egypt.ShereenEl-SayedPediatrics Department, Faculty of Medicine,
Ain Shams University, Cairo, Egypt.RagiaSaidPediatrics Department, Faculty of Medicine,
Ain Shams University, Cairo, Egypt.NesrineRadwanPediatrics Department, Faculty of Medicine,
Ain Shams University, Cairo, Egypt.MonaIsmailClinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, EgyptNaglaaAhmedPathology Department, Faculty of Medicine, Ain Shams University, Cairo, EgyptJournal Article20190822Background: Pediatric systemic lupus erythematosus (pSLE) accounts for<br />about 20% of all cases of Systemic Lupus Erythematosus (SLE), with<br />nephritis occurring in approximately 50% of the patients. Objective: to<br />evaluate the expression of CXCR3 in the kidneys and on CD4+ T cells in<br />pSLE. Methods: This study was conducted on 45 patients with pSLE<br />following up at the Allergy and Immunology Clinic, Children’s Hospital, Ain<br />Shams University and 45 age and sex matched healthy children as a control<br />group. Medical history, clinical examination and routine laboratory<br />investigations for assessment of disease activity were done for all patients,<br />the frequency of CXCR3, CD4+ T cells was determined in all patients and<br />controls. Twenty-five Paraffin blocks of patients with lupus nephritis (LN)<br />(available at the time of the study) underwent immunohistochemistry<br />staining for the frequencies of Chemokine C receptor (CXCR3). Results:<br />The absolute level and percentage of serum CD4+CXCR3+ were<br />significantly lower among our patients as compared to healthy controls. A<br />significant direct correlation was found between serum CD4+CXCR3+ and<br />both the lymphocytic count and quantitative Systemic Lupus erythematosus<br />disease activity index (SLEDAI), as well as a significant inverse correlation<br />between it and 24 hours urinary proteins. Variable degrees of CXCR3<br />expression seemed to have no impact on laboratory tests, British Isles Lupus<br />Assessment Group (BILAG) score and cumulative doses of<br />Immunosuppressives. Conclusion: Serum CD4+CXCR3+ and not renal<br />CXCR3 may be a potential marker of LN activity.https://ejpai.journals.ekb.eg/article_53991_04f3dcfd01f79d12316f5fb6d2870931.pdfThe Egyptian Society of Pediatric Allergy and Immunology (ESPAI)The Egyptian Journal of Pediatric Allergy and Immunology1687-164217220191001Can montelukast correct immune dysregulation in preschool children with mild persistent asthma?87955399310.21608/ejpa.2019.53993ENAmanyEl-KelanyDepartment of Pediatrics, faculty of
medicine, Suez Canal University, Ismailia,
Egypt.MahaAnaniClinical pathology department, faculty of
medicine, Suez Canal University, Ismailia,
Egypt.HananOmarClinical pathology, faculty of medicine, Suez Canal University, Ismailia,Egypt.AsmaaHashemMicrobiology and Immunology department, faculty of medicine, Suez Canal University, Ismailia, Egypt.0000-0002-0547-2096EnasFathyDepartment of Pediatrics, faculty of medicine, Suez Canal University, Ismailia,
Egypt.Journal Article20190416Background: Asthma is the most common inflammatory disorder among<br />preschool and school-age children. Regulation of immune cells and their<br />cytokines is essential to control asthma. Montelukast is a leukotriene<br />receptor antagonist that suppresses inflammatory cell proliferation, and<br />reduces cytokines and mediator secretion. Objective: The research<br />team's goal was to study the immunological parameters among mild<br />asthmatic patients before and after the treatment with Montelukast .<br />Methods: Forty preschool children with mild persistent asthma and<br />twenty healthy, non-allergic children were included in the study. Blood<br />eosinophil count, total IgE, serum IL-4, IL-10, and IL-13 levels were<br />assessed. T helper (CD3+CD4+) and T regulatory (CD4+CD25+) cell<br />counts were measured using flow cytometry; for mild asthmatics before<br />and after six weeks of treatment with Montelukast and for the control<br />group. Results: Asthmatic children have shown a significant elevation of<br />serum levels of IgE, IL4 and IL13, and also an increase of eosinophils,<br />total lymphocyte T cells and T helper cell count. However; serum levels<br />of IL10 and Treg cell count was lower in asthmatics compared to control.<br />Following six weeks of Montelukast treatment, all immunological<br />parameters improved. There was a significant elevation of serum levels<br />of IL10 and Treg cell count, with a decrease in serum levels of IgE, IL4<br />and IL13; eosinophil counts, and helper T cells. Conclusion:<br />Montelukast treatment improves the impaired immunological balance of<br />mild asthmatic children through the increase of serum IL-10, T<br />regulatory cell counts that have anti-inflammatory and<br />immunoregulatory effects. It also decreases T helper cells and their<br />proinflammatory cytokines.https://ejpai.journals.ekb.eg/article_53993_c4e7bb1f5af7c61ef80b0eb2b7a55a8f.pdf